Standard

ISO/TS 15216-2:2013 en;fr

Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection

  • This norm is withdrawn since 15-03-2013

128,05 139,57 Incl BTW

About norm

Status Withdrawn
Number of pages 29
Committee Microbiologie van de voedselketen
Published on 15-03-2013
Language English, French
This part of ISO/TS 15216 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Details

ICS-code 07.100.30
Dutch title Microbiologie van voedingsmiddelen en diervoeders - Horizontale methode voor de detectie van het hepatitis A virus en norovirus in voedingsmiddelen met gebruik van realtime RT-PCR - Deel 2: Methode voor kwalititatieve bepaling
English title Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection
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