Standard

NEN-EN 17280:2019 en

Foodstuffs - Determination of zearalenone and trichothecenes including deoxynivalenol and its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), nivalenol T-2 toxin and HT-2 toxin in cereals and cereal products by LC-MS/MS

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About norm

Status Current
Number of pages 48
Committee Voedingsmiddelenanalyse - Horizontale methoden
Published on 01-10-2019
Language English
NEN-EN 17280┬áspecifies a procedure for the determination of nivalenol (NIV), deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON), HT-2 and T-2 toxins (HT-2 and T-2) and zearalenone (ZEN) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) after clean-up by solid phase extraction (SPE). The method has been validated with samples of wheat, wheat flour, and wheat crackers. The wheat and the wheat flour were prepared from a mixture of wheat and fungi infected wheat kernels. The wheat crackers were baked from wheat flour and water spiked with the target mycotoxins. Validation levels for NIV ranged from 27,7 μg/kg to 378 μg/kg. Validation levels for DON ranged from 234 μg/kg to 2420 μg/kg. Validation levels for 3-acetyl-DON ranged from 18,5 μg/kg to 137 μg/kg. Validation levels for 15-acetyl-DON ranged from 11,4 μg/kg to 142 μg/kg. Validation levels for HT-2 ranged from 6,6 μg/kg to 134 μg/kg. Validation levels for T-2 ranged from 2,1 μg/kg to 37,6 μg/kg. Validation levels for ZEN ranged from 31,6 μg/kg to 230 μg/kg. Laboratory experiences have shown that this method is also applicable to barley and oat flour, and rye based crackers [5], however, this has not been validated in a collaborative study.

Details

ICS-code 67.060
Dutch title Voedingsmiddelen - Bepaling van zearalenone en trichothecenen, waaronder deoxynivalenol (DON) en de geacetyleerde derivaten ervan (3-acetyl-DON en 15-acetyl-DON), nivalenol (NIV) en T-2- en HT-2-toxine in granen en graanproducten met LC-MS/MS
English title Foodstuffs - Determination of zearalenone and trichothecenes including deoxynivalenol and its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), nivalenol T-2 toxin and HT-2 toxin in cereals and cereal products by LC-MS/MS
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