Norm

ASTM D4455-85(2014)

Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure

48,24

Over deze norm

Status Definitief
Aantal pagina's 3
Commissie D19.24
Gepubliceerd op 01-01-2014
Taal Engels

1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.

1.2 Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

1.3 The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.2

1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.

1.5 Approximately 104 cells/mL are required for detection by this test method.2

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Details

ICS-code 07.100.20
Nederlandse titel Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure
Engelse titel Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure

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