Norm

ASTM D7463 - 08 en

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water

  • Deze norm is ingetrokken sinds 01-05-2014

50,60 61,23 Incl BTW

Over deze norm

Status Ingetrokken
Aantal pagina's 7
Gepubliceerd op 01-12-2008
Taal Engels

1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with:

1.1.1 Microorganisms found in conventional liquid fuels with kinematic viscosities (at 40°C) of 8 mm2 · s1 as described in Table X6.1,

1.1.2 Microorganisms found in fuel-associated bottom water, and

1.1.3 Extracellular (non-cellular) ATP present in the sample matrix.

1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens and a dedicated luminometer and reported in (instrument specific) Relative Light Units.

1.3 This test method is equally suitable for use in the laboratory or field.

1.4 Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi.

1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 1011 M to 3 × 108 M which is equivalent to 1 × 1014 moles/mL to 3 × 1011 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 1011 M to 2 × 1014 M.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Details

ICS-code 07.100.99
Engelse titel Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
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