Norm

ASTM E1463-92(2012)

Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States

78,39

Over deze norm

Status Definitief
Aantal pagina's 23
Commissie E50.47
Gepubliceerd op 01-12-2012
Taal Engels

1.1 This guide describes procedures for obtaining data concerning the adverse effects of a test material (not food) added to marine and estuarine waters on certain species of marine and estuarine mysids during 96 h of continuous exposure. Juvenile mysids used in these tests are taken from cultures shortly after release from the brood and exposed to varying concentrations of a toxicant in static or flow-through conditions. These procedures will be useful for conducting toxicity tests with other species of mysids, although modifications might be necessary.

1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests with other species of mysids.

1.3 The procedures given in this guide are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications these procedures can be used to conduct acute tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, oil drilling fluids, produced water from oil well production, and other types of industrial wastes.

1.4 Results of acute toxicity tests with toxicants experimentally added to salt and estuarine waters should usually be reported in terms of a LC50 (median lethal concentration).

1.5 This guide is arranged as follows:

Section

Referenced Documents

2

Terminology

3

Summary of Guide

4

Significance and Use

5

Apparatus

6

 Facilities

6.1

 Construction Materials

6.2

 Metering Systems

6.3

 Test Chambers

6.4

 Cleaning

6.5

 Acceptability

6.6

Safety Precautions

7

Dilution Water

8

 Requirements

8.1

 Source

8.2

 Treatment

8.3

 Characterization

8.4

Test Material

9

 General

9.1

 Stock Solution

9.2

 Test Concentrations

9.3

Test Organisms

10

 Species

10.1

 Age

10.2

 Source

10.3

 Brood Stock

10.4

 Food

10.5

 Handling

10.6

 Harvesting Young

10.7

 Quality

10.8

Procedure

11

 Experimental Design

11.1

 Dissolved Oxygen

11.2

 Temperature

11.3

 Loading

11.4

 Salinity

11.5

 Light

11.6

 Beginning of Test

11.7

 Feeding

11.8

 Duration of Test

11.9

 Biological Data

11.10

 Other Measurements

11.11

Analytical Methodology

12

Acceptability of Test

13

Interpretation of Results

14

Report

15

Appendixes

Holmesimysis costata

X1

Neomysis mercedis

X2


1.6 The values stated in SI units are to be regarded as the standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.

Details

ICS-code 65.100.99
Nederlandse titel Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States
Engelse titel Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States

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