Over deze norm
This part of the CEN Technical Report specifies a membrane filtration procedure for the quantitative resuscitation and enumeration, by culture of individual colonies on chromogenic agar media, of Salmonella spp. including potentially sub-lethally damaged Salmonella spp. in sewage sludges. It may be suitable for other sludges, soils, soil improvers, growing media and biowastes but the user shall validate the method using these materials. The fully defined scope will be determined after the proposed validation trials have been agreed and carried out. The method is particularly suited to determining the efficiency of treatment procedures for the elimination of pathogens in sewage sludge as outlined in the Revision of Directive 86/278/EEC (3rd Draft, CEN/TC 308 - doc 525). Treatment type A processes are initially to be validated through a to be defined Log10 reduction with a test organism such as Salmonella senftenberg W775. The method has a limit of detection of approximately 1 cfu/g wet weight sludge, dependent on the solids content which at high concentrations (> 20 % (w/v)) can restrict filtration of the sample volume through the membrane if not first diluted.
|Nederlandse titel||Karakterisering van slib - Detectie en telling van Salmonella spp. in slib, grond, bodemverbeterende middelen, teeltsubstraten en bioafval - Deel 1: Membraanfiltratiemethode voor kwantitatieve resuscitatie van subletaal beschadigde bacteriÃ«n (om de doeltreffendheid van behandelingsmethoden te staven in logaritmische afneming)|
|Engelse titel||Characterization of sludges - Detection and enumeration of Salmonella spp. in sludges, soils, soil improvers, growing media and biowastes - Part 1: Membrane filtration method for quantitative resuscitation of sub-lethally stressed bacteria (to confirm efficacy of log drop treatment procedures)|