ISO/DIS 16266-2:2017 en

Water - Detectie en telling van Pseunomonas aeruginosa - Deel 2: "Meest waarschijnlijke aantal" methode

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Status Ontwerp
Aantal pagina's 53
Gepubliceerd op 25-03-2017
Taal Engels
This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The method is based on the growth of target organisms in a liquid medium and calculation of the “Most Probable Number” (MPN) of organisms by reference to MPN tables. This method can be applied to a range of types of water, for example, hospital waters, drinking water and non-carbonated bottled waters intended for human consumption, groundwater, distilled water, swimming pool and spa pool waters and cooling tower waters including those containing high background counts of heterotrophic bacteria (see References [4] and [5]). This method is not suitable for carbonated bottled waters. The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a 7-amido-coumarin aminopeptidase substrate present in the reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the Pseudalert1) reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the 7-amido-coumarin aminopeptidase substrate releasing a product which fluoresces under ultraviolet (UV) light. The test described in this document provides a confirmed result within 24 h with no requirement for further confirmation of positive wells.


ICS-code 13.060.70
Nederlandse titel Water - Detectie en telling van Pseunomonas aeruginosa - Deel 2: "Meest waarschijnlijke aantal" methode
Engelse titel Water quality - Detection and enumeration of Pseudomonas aeruginosa - Part 2: Most probable number method



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