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This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strain Arxula adeninivorans. Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %. This method is applicable to: fresh water; waste water; sea water; brackish water; aqueous extracts and leachates; eluates of sediments (fresh water); pore water; aqueous solutions of single substances or of chemical mixtures; drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng/l and 3 ng/l 17β-estradiol equivalents (EEQ). The upper threshold of the dynamic range for this test is between 25 ng/l and 40 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.
||Water - Bepaling van de oestrogeen belasting van water en afvalwater - Deel 2: Gist oestrogeen screening (A-YES, Arxula adeninivorans)
||Water quality - Determination of the estrogenic potential of water and waste water - Part 2: Yeast estrogen screen (A-YES, Arxula adeninivorans)