Norm

NEN-EN 17425:2019 Ontw. en

Voedingsmiddelen - Bepaling van van ergot-alkaloiden in granen en graanproducten met LC-MS/MS en zuivering met dispersieve SPE

36,00 43,56 Incl BTW

Over deze norm

Status Ontwerp
Aantal pagina's 44
Commissie Voedingsmiddelenanalyse - Horizontale methoden
Gepubliceerd op 01-08-2019
Taal Engels
This document describes a method for the determination of the sum total of six ergot alkaloids (ergocornine, ergometrine, ergocristine, ergotamine, ergosine and ergocryptine) and their -inine epimer pairs by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) after clean-up by dispersive solid phase extraction (SPE). The method has been validated for cereals and cereal-based food products. The method has been validated in the range 13,2 μg/kg to 168 μg/kg for the sum of the twelve ergot alkaloids, in rye flour, rye bread and cereal products (breakfast cereal, infant breakfast cereal, and crispbread) that contained rye as an ingredient, as well as seeded wholemeal flour and a barley and rye flour mixture. Method performance was satisfactory in the range 24,1 μg/kg to 168 μg/kg, however at lower concentrations RSDR values were greater than 44 %, and HorRat values exceeded 2,0, indicating the method may not be fully suitable at concentrations below 24 μg/kg for sum of ergot alkaloids, although it is suitable for screening at these concentrations. Method performance may be improved by inclusion of an isotopically labelled internal standard, but this was not available at the time of the method validation study.

Details

ICS-code 67.050
67.060
Nederlandse titel Voedingsmiddelen - Bepaling van van ergot-alkaloiden in granen en graanproducten met LC-MS/MS en zuivering met dispersieve SPE
Engelse titel Foodstuffs - Determination of ergot alkaloids in cereals and cereal products by dSPE clean-up and LC-MS/MS

Winkelwagen

Subtotaal:

Ga naar winkelwagen