Norm

NEN-EN-ISO 17601:2018 en

Bodem - Schatting van de talrijkheid van geselecteerde microbiële gensequenties door kwantitatieve real-time PCR van rechtstreeks uit de bodem geëxtraheerd DNA

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Over deze norm

Status Definitief
Aantal pagina's 31
Commissie Milieukwaliteit
Gepubliceerd op 01-03-2018
Taal Engels
NEN-EN-ISO 17601 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups. It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Details

ICS-code 13.080.30
Nederlandse titel Bodem - Schatting van de talrijkheid van geselecteerde microbiële gensequenties door kwantitatieve real-time PCR van rechtstreeks uit de bodem geëxtraheerd DNA
Engelse titel Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

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