Norm

NPR-CEN/TS 15633-3:2012 en

Voedingsmiddelen - Detectie van allergenen in voeding met behulp van immunologische methoden - Deel 3: Screening voor de aanwezigheid van hazelnoot met een enzym immunoassay methode

61,30

Over deze norm

Status Definitief
Aantal pagina's 33
Gepubliceerd op 01-08-2012
Taal Engels
This Technical Specification specifies an enzyme-linked immunosorbent assay (ELISA)-method for the determination of hazelnut concentration in food samples. Spiking experiments with diluted ground hazelnut have been used to validate the method’s use on food matrices such as mixed grain cereals, dark chocolate (45 % cocoa) and ice cream. The range of the method is 0,5 mg to 5,0 mg hazelnut protein per kg of food sample. As hazelnut kernels typically contain between 12 % to 15 % protein, this equates to approximately 3,7 mg to 37 mg hazelnut kernel per kg of food sample. The upper limit of the range of quantitation can be extended, if required, by further dilution of sample extracts. The method is commercially available) and has been validated in-house by the manufacturer. These data are included in Annex A.2. The method has been successfully validated by a collaborative study. The study was organized by the Working Group established by the Federal Office of Consumer Protection and Food Safety (BVL) for the execution of § 64 of the German Food and Feed Code (LFGB) for the determination of hazelnut content in dark chocolate. Thirteen German laboratories participated in the collaborative study. These data are included in Annex A.3.

Details

ICS-code 67.050
Nederlandse titel Voedingsmiddelen - Detectie van allergenen in voeding met behulp van immunologische methoden - Deel 3: Screening voor de aanwezigheid van hazelnoot met een enzym immunoassay methode
Engelse titel Foodstuffs - Detection of food allergens by immunological methods - Part 3: Quantitative determination of hazelnut with an enzyme immunoassay using polyclonal antibodies and Lowry protein detection

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