Norm

NPR-ISO/TS 21569-2:2012 en

Horizontale methode voor moleculaire biomarkeranalyse - Analysemethodevoor de detectie van genetisch gemodificeerde organismen en afgeleide producten - Deel 2: PCR methode voor signaleren van FP967 pieken in lijnzaad en lijnzaadproducten

52,53

Over deze norm

Status Definitief
Aantal pagina's 9
Commissie Beleidscommissie Landbouw & Levensmiddelen
Gepubliceerd op 01-10-2012
Taal Engels
This method describes a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as "CDC Triffid"). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopalin synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli. The method described is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.

Details

ICS-code 67.050
Nederlandse titel Horizontale methode voor moleculaire biomarkeranalyse - Analysemethodevoor de detectie van genetisch gemodificeerde organismen en afgeleide producten - Deel 2: PCR methode voor signaleren van FP967 pieken in lijnzaad en lijnzaadproducten
Engelse titel Horizontal methods for molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products

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