Norm

NVN-ISO/TS 20224-3:2020 en

Molecular biomarker analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Part 3: Porcine DNA detection method

88,37 96,32 Incl BTW

Over deze norm

Status Definitief
Aantal pagina's 17
Commissie Biomarkers
Gepubliceerd op 01-08-2020
Taal Engels
NVN-ISO/TS 20224-3 specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of porcine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of porcine material derived from pig (Sus scrofa domesticus) and wild boar (Sus scrofa).The target sequence is a partial fragment of the Sus scrofa beta actin (ACTB) gene, partial cds. (i.e. GenBank accession number DQ452569.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).

Details

ICS-code 67.050
Engelse titel Molecular biomarker analysis - Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Part 3: Porcine DNA detection method

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